“The transition from 2D to 3D spatial omics is transformative for neuroscience. While 2D methods provide molecular profiles, they miss critical long-range cellular interactions. We can now visualize these connections at an unprecedented scale, enabling high-throughput analysis of genetic perturbations across complex tissues. Combining CRISPR gene editing with high-resolution spatial analysis allows us to uncover new insights into brain development and disease progression in ways we never could before.”